A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w+mC and the mini-yellow marker y+mDint2, and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
The P{EPgy2} construct is similar to P{EP}, but incorporates a modification that allows expression driven by the Scer\GAL4 regulatory sequences to occur in the female germline (seeFBrf0128569). Mobilization of the P-element occurred in animals carrying P{EPgy2} on a second chromosome CyO balancer chromosome and P{Δ2-3}99B, a stable source of transposase, on a marked third chromosome. Genomic locations of new insertions were determined by assessment of flanking sequence by high-throughput methods and balanced stocks were created.
Flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence; 2338 lines were selected for inclusion in the BDGP Gene Disruption Project 2004 collection.
An additional 11,830 P{EPgy2} insertion lines were generated and 9585 were mapped to unique sites; 1193 were selected for addition to the BDGP Gene Disruption Project collection.
