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General Information
Name
TI_set_P{wHy}.DG
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000514
Vector
    Title
    A set of P-element construct P{wHy} insertions.
    Accessions
      Overview
      Description

      A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{wHy}. The P{wHy} construct carries two visible markers, the mini-white marker w+mC and the yellow marker y+t7.7, which flank a transposition-competent hobo element. It was designed as a deletion generation and detection system: exposure to the hobo transposase results local deletions and concomitant loss of one marker or the other.

      Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection; some lines were mapped by the GDP.

      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Transgenic Construct used
      Protocol

      A genetic screen allowing selection of new autosomal insertions was used. Parental males carried a P{wHy} insertion on the X chromosome and a transposase-producing construct.

      Markers in stocks (as originally submitted to the BDSC):y1 w67c23.

      Mode of Assay
      Data analysis

      Flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence; 154 lines were selected for inclusion in the BDGP Gene Disruption Project 2004 collection.

      Genomic sequences flanking the insertions were determined by the Universal Fast Walking method (FBrf0216132).

      Comments
      Associated Data
      Size
      Associated features
      820 Insertion(s)
      Files
      Additional Information
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      GDP_insertions_P{wHy}.DG
      TI_set_P{wHy}.DG
      TI_set_P{wHy}.Gelbart
      Name Synonyms
      A set of P-element construct P{wHy} insertions.
      Secondary FlyBase IDs
        References (7)