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General Information
Name
TI_set_PBac{5HPw[+]}.PA
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000515
Vector
    Title
    A set of Tni\piggyBac-based construct PBac{5HPw+} insertions.
    Accessions
      Overview
      Description

      PBac{5HPw+} is a 5' half P-element construct marked with the w+mC mini-white marker nested within a Tni\piggyBac-based construct; mobilization requires a source of Tni\piggyBac transposase.

      A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{5HPw+}.

      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Transgenic Construct used
      Protocol

      Genetic screens allowing selection of new autosomal insertions were carried out. Two different homozygous viable and fertile X chromosome insertions were used as the source of the PBac{5HPw+} construct to be mobilized.

      Markers in stocks (as originally submitted to the BDSC):y1 w1118.

      Mode of Assay
      Data analysis

      Flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence. 342 lines from the combined PA and PC collections were selected for inclusion in the BDGP Gene Disruption Project 2004 collection.

      Comments

      One of several datasets using a Tni\piggyBac-based construct rather than a P-element construct that were included in the GDP collection, in order to broaden the spectrum of insertion sites.

      Associated Data
      Size
      Associated features
      239 Insertion(s)
      Files
      Additional Information
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      GDP_insertions_PBac{5HPw+}.PA
      TI_set_PBac{5HPw+}.PA
      TI_set_PBac{5HPw+}.Ring_Garza
      Name Synonyms
      A set of Tni\piggyBac-based construct PBac{5HPw+} insertions.
      Secondary FlyBase IDs
        References (6)