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General Information
Name
TI_set_P{XP}.Exel.d
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000520
Vector
    Title
    A set of P-element construct P{XP} insertions.
    Accessions
      Overview
      Description

      A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{XP}. The P{XP} construct carries the w+mC mini-white marker, long (199-bp) Scer\FRT sites, and is designed to allow flexible use of Scer\UAS sites for Scer\GAL4-driven misexpression of adjacent genes. FRT sites allow Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.

      P{XP} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.

      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Transgenic Construct used
      Protocol

      The P{XP} element used for mobilization was located on a first-chromosome balancer Binsinscy; the transposase source was P{Δ2-3}99B, a stable source of transposase on the third chromosome. Dysgenic females were used; from the dysgenic crosses a single new insertion was retained per vial, to avoid premeiotic clusters. Insertions were mapped to a chromosome by standard genetic methods, examined for homozygous viability and used for recovery of flanking genomic sequence.

      Markers in stocks (as originally submitted to the BDSC):w1118.

      Mode of Assay
      Data analysis

      Flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence. For the combined Exelixis lines, 89% could be uniquely mapped to the genome.

      Reanalysis of the genomic mapping of most of the combined Exelixis collections (insertions of P{XP}, PBac{PB}, PBac{RB}, and PBac{WH}) was undertaken. After multiple assessments, including sequencing and alignment of inverse PCR products, 16,073 insertions were mapped to unique sites. Of these, 1859 lines were added to the Gene Disruption Project collection.

      Comments
      Associated Data
      Size
      Associated features
      6,134 Insertion(s)
      Files
      Additional Information
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      GDP_insertions_P{XP}.Exelixis
      TI_set_P{XP}.Exel.d
      Name Synonyms
      A set of P-element construct P{XP} insertions.
      Secondary FlyBase IDs
        References (6)