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General Information
Name
TI_set_PBac{WH}.Exel.f
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000523
Vector
    Title
    A set of Tni\piggyBac-based construct PBac{WH} insertions.
    Accessions
      Overview
      Description

      A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{WH}. The PBac{WH} construct carries the w+mC mini-white marker, a single long (199-bp) Scer\FRT site, and a terminal Scer\UAS site for Scer\GAL4-driven misexpression of adjacent genes. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.

      PBac{WH} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.

      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Transgenic Construct used
      Protocol

      The PBac{WH} element was mobilized using P{Tub-PBac\T} as the source of Tni\piggyBac transposase. All stocks used for creation and characterization of new insertions were in an isogenic w[-] background. Both dysgenic males and females were used; from the dysgenic crosses a single new insertion was retained per vial, to avoid premeiotic clusters. Insertions were mapped to a chromosome by standard genetic methods, examined for homozygous viability and used for recovery of flanking genomic sequence.

      Markers in stocks (as originally submitted to the BDSC):w1118.

      Mode of Assay
      Data analysis

      Flanking genomic sequences from both 5′ and 3′ piggyBac ends were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence. For the combined Exelixis lines, 89% could be uniquely mapped to the genome.

      Reanalysis of the genomic mapping of most of the combined Exelixis collections (insertions of P{XP}, PBac{PB}, PBac{RB}, and PBac{WH}) was undertaken. After multiple assessments, including sequencing and alignment of inverse PCR products, 16,073 insertions were mapped to unique sites. Of these, 1859 lines were added to the Gene Disruption Project collection.

      Comments
      Associated Data
      Size
      Associated features
      6,649 Insertion(s)
      Files
      Additional Information
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      GDP_insertions_PBac{WH}.Exelixis
      TI_set_PBac{WH}.Exel.f
      Name Synonyms
      A set of Tni\piggyBac-based construct PBac{WH} insertions.
      Secondary FlyBase IDs
        References (6)