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General Information
Name
TI_set_Mi{ET1}.MB
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000524
Vector
    Title
    A set of Dhyd\Minos-based construct Mi{ET1} insertions.
    Accessions
      Overview
      Description

      A set of transgenic insertion stocks derived by TE mobilization using the Dhyd\Minos-based construct Mi{ET1}; vetted by the Gene Disruption Project (GDP). The Mi{ET1} construct carries the Avic\GFPE.3xP3 fluorescent marker, a Scer\GAL4 driver/reporter gene, and bacterial sequences that allow plasmid rescue.

      Additional Mi{ET1} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.

      Available from
      Bloomington Drosophila Stock Center
      Not all insertions in this dataset are available as extant stocks.
      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Transgenic Construct used
      Protocol

      A genetic screen allowing recovery of insertions on all chromosomes was carried out in a w1118 isogenic background (FBrf0179424). Mobilization of the Minos element occurred in animals carrying Mi{ET1} on TM3-SbSer, a third chromosome balancer chromosome and P{hsILMiT}2.4, a stable source of Minos transposase, on an second chromosome balancer chromosome, CyO-MiT2.4.

      Markers in stocks (as originally submitted to the BDSC):w1118;y1 w67c23.

      Mode of Assay
      Data analysis

      Flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence. 10,781 insertions from 10,630 strains were mapped to a unique site in the genome. Lines selected for the Gene Disruption Project collection were balanced and their insertions sites verified by resequencing. Stocks were generated and balanced in the w1118 isogenic background.

      Comments
      Associated Data
      Size
      Associated features
      2,727 Insertion(s)
      Files
      Additional Information
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      GDP_insertions_Mi{ET1}.MB
      TI_set_Mi{ET1}.MB
      Name Synonyms
      A set of Dhyd\Minos-based construct Mi{ET1} insertions.
      Secondary FlyBase IDs
        References (6)