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General Information
Name
TI_set_P{EP}.G
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000525
Vector
    Title
    A set of P-element construct P{EP} insertions.
    Accessions
      Overview
      Description

      A set of transgenic insertion stocks derived by TE mobilization using the using the P-element construct P{EP}. The P{EP} construct construct carries a w+mC mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue (FBrf0090768). The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.

      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Transgenic Construct used
      Protocol

      Markers in stocks (as originally submitted to the BDSC):w*; w1118 ; y1 w*.

      Mode of Assay
      Data analysis

      1685 strains produced in a large-scale screen by GenExel (now Aprogen) were evaluated; flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence. 1136 selected lines were balanced and added to the Gene Disruption Project collection.

      Comments

      The Scer\GAL4 driver in the P{EP} construct cannot be activated in the female germ line (FBrf0128569).

      Associated Data
      Size
      Associated features
      1,337 Insertion(s)
      Files
      Additional Information
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      GDP_insertions_P{EP}.GenExel
      TI_set_P{EP}.G
      Name Synonyms
      A set of P-element construct P{EP} insertions.
      Secondary FlyBase IDs
        References (5)