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General Information
Name
TI_set_P{EPg}.HP
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000528
Vector
    Title
    A set of P-element construct P{EPg} insertions.
    Accessions
      Overview
      Description

      A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPg}. The P{EPg} construct carries the mini-white marker w+mC, and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator (FBrf0128569). The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.

      Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Transgenic Construct used
      Protocol

      More than 8,500 independent insertions were generated.

      Markers in stocks (as originally submitted to the BDSC):w1118.

      Mode of Assay
      Data analysis

      Flanking genomic sequences were determined by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence.

      Lines donated by DeveloGen (now Evotec) were added to the Gene Disruption Project collection.

      Comments

      P{EPg} is similar to P{EP}, but has been modified to allow activation of the Scer\GAL4 driver in the female germline (FBrf0128569).

      Associated Data
      Size
      Associated features
      378 Insertion(s)
      Files
      Additional Information
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      GDP_insertions_P{EPg}.HP
      TI_set_P{EPg}.Gottingen
      TI_set_P{EPg}.HP
      Name Synonyms
      A set of P-element construct P{EPg} insertions.
      Secondary FlyBase IDs
        References (6)