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General Information
Name
TI_set_P{lacW}.G
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000529
Vector
    Title
    A set of lethal or semi-lethal mutations associated with insertion of the P-element construct P{lacW} insertions.
    Accessions
      Overview
      Description

      A set of X chromosome mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or semi-lethal phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

      Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Protocol

      A genetic screen allowing selection of lethal or semi-lethal insertions on the X chromosome was carried out. Mobilization of the P{lacW} element occurred in males carrying a w[-] allele on the X chromosome, P{lacW} on the second chromosome and P{Δ2-3}99B, a stable source of P transposase, on a marked third chromosome. The selection scheme produced balanced stocks of X chromosome insertions; 501 lines that were hemizygously lethal or semi-lethal were established.

      Markers in stocks (as originally submitted to the BDSC):w67c23.

      Mode of Assay
      Data analysis

      Flanking genomic sequences for 496 lines were obtained by sequencing of plasmid rescued or inverse PCR products, and were mapped by alignment to the genomic sequence. The cytological insertion sites of 401 strains were determined by in situ hybridization on polytene chromosome squashes. These analyses indicated that >90% of the lines contained single insertions.

      Comments
      Associated Data
      Size
      Associated features
      540 Insertion(s)
      Files
      Additional Information
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      GDP_insertions_P{lacW}.Gottingen
      Gottingen collection
      TI_set_P{lacW}.G
      TI_set_P{lacW}.Gottingen
      Name Synonyms
      A set of lethal or semi-lethal mutations associated with insertion of the P-element construct P{lacW} insertions.
      Secondary FlyBase IDs
        References (6)