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General Information
Name
TI_set_P{lacW}.SH
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000530
Vector
    Title
    A set of mutations associated with insertion of the P-element construct P{lacW} insertions.
    Accessions
      Overview
      Description

      A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

      Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Protocol

      A genetic screen allowing selection new insertions on the autosomes was carried out. Mobilization of the P{lacW} element occurred in males carrying a w[-] allele and P{lacW} on the X chromosome, and P{Δ2-3}99B, a stable source of P transposase, on a marked third chromosome. A singe w[+] F1 male from each dysgenic cross was used for subsequent characterization, to avoid pre-meiotic clusters. Lethal insertions were selected and balanced stocks created. 2355 second chromosome insertion lines and 160 third chromosome insertion lines were retained for further characterization.

      Markers in stocks (as originally submitted to the BDSC):w*.

      Mode of Assay
      Data analysis

      Flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence. A final set of 854 independent insertion lines was selected for extensive characterization, representing 783 genes on II and 67 genes on III.

      Comments
      Associated Data
      Size
      Associated features
      938 Insertion(s)
      Files
      Additional Information
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      GDP_insertions_P{lacW}.SH
      TI_set_P{lacW}.SH
      TI_set_P{lacW}.Szeged_Hou
      Name Synonyms
      A set of mutations associated with insertion of the P-element construct P{lacW} insertions.
      Secondary FlyBase IDs
        References (6)