Mobilization of the P-element occurred in animals carrying P{RS3} on a second chromosome SM6a balancer chromosome and P{Δ2-3}99B, a stable source of transposase, on a marked third chromosome. A second set of mobilization experiments used dysgenic males carrying P{RS3} on the Y chromosome and P{Δ2-3}99B on the third chromosome. Stocks were generated and balanced in an w¹¹¹⁸ isogenic background.

Flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence.