FB2021_06, released December 14, 2021

Reagent: TI_set_P{RS3}.DrosDel

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General Information
Name
TI_set_P{RS3}.DrosDel
Species
D. melanogaster
Reagent type
transgenic insertion collection
FlyBase ID
FBlc0000535
Project
Created by
Vector
    Title
    A set of P-element construct P{RS3} insertions.
    Accessions
      Overview
      Description

      A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{RS3}. The P{RS3} construct carries a mini-white marker and FRT recombination sites (FBrf0091061); it serves as one recombination endpoint in the DrosDel deletion-generation system.

      (Ryder et al., 2004)
      Available from
      Drosophila Genetic Resource Center, Kyoto, Japan
      Most insertions in this dataset are available as extant stocks.
      (FlyBase Curators, 2013)
      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Transgenic Construct used
      Protocol

      Mobilization of the P-element occurred in animals carrying P{RS3} on a second chromosome SM6a balancer chromosome and P{Δ2-3}99B, a stable source of transposase, on a marked third chromosome. A second set of mobilization experiments used dysgenic males carrying P{RS3} on the Y chromosome and P{Δ2-3}99B on the third chromosome. Stocks were generated and balanced in an w1118 isogenic background.

      (Ryder et al., 2004)
      Mode of Assay
      Data analysis

      Flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence.

      (Ryder et al., 2004)
      Comments
      Associated Data
      Size
      Associated features
      1,692 Insertion(s)
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      Files
      Additional Information
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      TI_set_P{RS3}.DrosDel
      (Schroeder, 2014.6.12)
      Name Synonyms
      A set of P-element construct P{RS3} insertions.
      (FlyBase Curators, 2016-)
      Secondary FlyBase IDs
        References (4)