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General Information
Name
TI_set_P{GSV1}.DGSP
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000574
Vector
    Title
    A set of P-element construct P{GSV1} insertions.
    Accessions
      Overview
      Description

      A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GSV1}. The P{GSV1} construct construct carries a w+mC mini-white visible marker; it carries two sets Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator plus a core promoter, one adjacent to each terminus and oriented to direct transcription outward. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.

      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Transgenic Construct used
      Protocol

      Mobilization of the P-element occurred in animals carrying P{GSV1} on a second chromosome CyO balancer chromosome and P{Δ2-3}99B, a stable source of P transposase, on a marked third chromosome. Stable homozygous or balanced stocks were created.

      Mode of Assay
      Data analysis

      For the initial collection, 613 stable lines were crossed to 4 different GAL4-expressing lines; F1 individuals carrying both a GAL4 transgene and a P{GSV1} insert were screened for lethality and visible phenotypes. GAL4 expression patterns were observed using the P{UAS-GFP.S65T} reporter construct. For a subset of 170 lines, the identity of the induced transcript(s) was determined by RT-PCR, using vector-specific and oligo-dT primers.

      Comments
      Associated Data
      Size
      Associated features
      787 Insertion(s)
      Files
      Additional Information
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      TI_set_P{GSV1}.DGSP
      Name Synonyms
      A set of P-element construct P{GSV1} insertions.
      Secondary FlyBase IDs
        References (5)