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General Information
Name
TI_set_P{GawB}.NP
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000576
Project
    Created by
    Vector
      Title
      A set of P-element construct P{GawB} insertions.
      Accessions
        Overview
        Description
        A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.
        Biosample Source
        Overview
        Strain
        Stage
        Sex
        Tissue isolated
        Other tissues studied
        Cell component
        Cell line
        Key genes
        Methods
        Sample preparation
        Reagent Details
        Methods
        Transgenic Construct used
        Protocol
        Mobilization of the P-element occurred in males carrying P{GawB} on a second chromosome CyO balancer chromosome and a source of P transposase. Stable homozygous or balanced stocks were created; insertions were recovered on all chromosomes.
        Mode of Assay
        Data analysis
        Flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence; 3,825 insertions mapped to unique euchromatic locations. An additional 143 insertions were mapped by plasmid rescue.
        Comments
        Associated Data
        Size
        Associated features
        3,874 Insertion(s)
        Files
        Additional Information
        Synonyms and Secondary IDs (3)
        Reported As
        Symbol Synonym
        Name Synonyms
        A set of P-element construct P{GawB} insertions.
        Secondary FlyBase IDs
          References (4)