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General Information
Name
Stau_RIP
Species
D. melanogaster
Result type
FlyBase ID
FBlc0000670
Project
Data Provider
Title
High-confidence RNA-protein interactions identified in stau and stau-GFP RIP from early embryos.
Status
Current
Accessions
    Biosample Source
    Overview
    Strain
    Sex
    Tissue isolated
    Other tissues studied
    Cell component
    Cell line
    Key genes
    (transgene_used)
    Methods
    Sample preparation

    Flies were maintained at 25oC and a 0-3 hour embryo collection was collected.

    Biosamples analyzed by this result (2)
    Biosample
    Type
    Title
    D. melanogaster, stau-GFP line in stau mutant background, embryo (0-3 hr), source of extract for stau-GFP RIP.
    D. melanogaster, wild-type, embryo (0-3hr), source of extract for stau RIP.
    Data Analyzed
    Assay methods
    Key genes
    (bait_protein)
    Protocol

    The stau protein was immunoprecipitated using a synthetic FLAG-tagged Fab antibody fragment specific for stau. Bound protein-RNA was eluted using FLAG peptide. RNA in coimmunoprecipitation was purified using Trizol reagent. Double-stranded cDNA was prepared from isolated RNA using oligo-dT primers and labelled for subsequent hybridization.

    Mode of Assay

    Labelled cDNA libraries were characterized by hybridization to custom-designed NimbleGen array (GPL10539).

    Raw Data Analyzed (2)
    Assay / Reagent collection
    Type
    Title
    Immunoprecipitation of RNAs (RIP) bound to stau in early embryos.
    Immunoprecipitation of RNAs (RIP) bound to GFP-tagged stau in early embryos.
    Processed Data Analyzed (0)
    Result
    Type
    Title
    Analysis
    Methods
    Reference Genome
    Reference Annotation
    Data analysis

    Interactions reported for RNA recovered in stau RIP with > 5-fold enrichment (GFP-stau RIP) or >2-fold enrichment (stau RIP), with FDR of 5%.

    Comments
    Associated Data
    Size
    Associated interactions
    94 Interactions
    Files
    Additional Information
    Synonyms and Secondary IDs (3)
    Reported As
    Symbol Synonym
    Stau_RIP
    Staufen-associated mRNAs
    Name Synonyms
    High-confidence RNA-protein interactions identified in stau and stau-GFP RIP from early embryos.
    Secondary FlyBase IDs
      References (1)