Open Close
General Information
Name
ChIP-seq_RpII33_E6-8h_mesoderm
Species
D. melanogaster
Result type
FlyBase ID
FBlc0000709
Project
Data Provider
[Furlong lab, EMBL](http://furlonglab.embl.de)
Title
Chip-Seq peak calls for Pol II (RpII33) from D. melanogaster, embryonic mesodermal nuclei (6-8 hr AEL).
Status
Current
Accessions
    Biosample Source
    Overview
    Strain
    Sex
    Tissue isolated
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Sample preparation

    A strain expressing mesodermally expressed SBP-tagged His2B was used to label nuclei for subsequent fluorescent "cell" sorting. Two independent biological replicates were used.

    Biosamples analyzed by this result (1)
    Biosample
    Type
    Title
    D. melanogaster, embryonic mesodermal nuclei (6-8 hr AEL), source for chromatin.
    Data Analyzed
    Key genes
    (bait_protein)
    Protocol

    Chromatin immunoprecipitation of Pol II subunit RpII33.

    Chromatin was prepared from formaldehyde-fixed embryos as previously described (FBrf0202481).

    Mode of Assay

    Chromatin immunoprecipitated DNA was analyzed by next generation sequencing.

    Raw Data Analyzed (1)
    Assay / Reagent collection
    Type
    Title
    Chip-Seq of Pol II (RpII33) from D. melanogaster, embryonic mesodermal nuclei (6-8 hr AEL).
    Processed Data Analyzed (0)
    Result
    Type
    Title
    Analysis
    Methods
    Reference Genome
    Reference Annotation
    Data analysis

    Reads were aligned using bowtie. Input DNA or His3 ChIP DNA was used as control data. Peaks were called with MACS 1.3.7.1. Peak calls were lifted over from Dmel_Release_5 to Dmel_Release_6 using the NCBI Genome Remapping tool.

    Comments
    Associated Data
    Size
    Associated features
    5,328 region(s)
    Additional Information
    Synonyms and Secondary IDs (4)
    Reported As
    Symbol Synonym
    ChIP-seq_RpII33_E6-8h_mesoderm
    Pol II BiTS ChIP
    Pol II Enriched Regions at 6-8h AEL in Mesoderm
    Name Synonyms
    Chip-Seq peak calls for Pol II (RpII33) from D. melanogaster, embryonic mesodermal nuclei (6-8 hr AEL).
    Secondary FlyBase IDs
      References (2)