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General Information
Name
VDRC-VT
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000718
Project
Created by
[Dickson group](https://www.janelia.org/lab/dickson-lab) [Stark group at IMP, Vienna](http://www.imp.ac.at/research/research-groups/stark-group/)
Vector
Title
A set of GAL4 transgenes under the control of putative enhancer fragments.
Accessions
    Overview
    Description

    A collection of stocks carrying GAL4 transgenes fused to putative enhancer fragments from selected D. melanogaster genes; a targeted insertion site is used.

    Available from
    Biosample Source
    Overview
    Strain
    Stage
    Sex
    Tissue isolated
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation
    Reagent Details
    Methods
    Molecular Construct used
    Transgenic Construct used
    Protocol

    7,705 candidate enhancer sequences were PCR amplified from genomic DNA, covering 13.5% of the entire non-coding and non-repetitive genome; genomic fragments were 2kb on average, and typically overlapped ~400bp to avoid splitting enhancers. Candidate enhancer fragments were cloned into the pBPGUw vector, upstream of a Drosophila synthetic core promoter (DSCP) that contains the TATA, Inr, MTE, and DPE sequence motifs, and a Scer\GAL4 reporter gene. These constructs were integrated into the P{CaryP}attP2 target element on chromosome 3L by phiC31 site-specific integration, as described elsewhere (FBrf0205679).

    Mode of Assay
    Data analysis
    Comments
    Associated Data
    Size
    Associated features
    5,199 region(s)
    3,604 regulatory_region(s)
    Files
    Additional Information

    [Vienna Tiles GAL4 Library](http://stockcenter.vdrc.at/control/library_vt)

    Synonyms and Secondary IDs (5)
    Reported As
    Symbol Synonym
    VDRC-VT
    VT collection
    VT library
    Vienna Tiles
    Name Synonyms
    A set of GAL4 transgenes under the control of putative enhancer fragments.
    Secondary FlyBase IDs
      References (3)