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General Information
Name
RPCI-98_BAC
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000777
Project
Created by
Vector
    Title
    BAC genomic clone collection for D. melanogaster, iso-1 strain, adult, 163kb inserts.
    Accessions
    Overview
    Description
    A BAC library containing genomic DNA fragments with average size of 163 kb. Generated for physical mapping of the Drosophila genome.
    Available from
    Biosample Source
    Overview
    Sex
    Tissue isolated
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation
    Biosamples used (1)
    Biosample
    Type
    Title
    D. melanogaster, iso-1 strain, adult, nucleus, source for BAC genomic clones.
    Reagent Details
    Protocol
    DNA was isolated from adult fly tissue and embedded in agarose blocks. DNA was partially digested with EcoRI and EcoRI methylase, size fractionated and cloned into the pBACe3.6 vector.
    Mode of Assay
    Data analysis
    Comments
    A total of 17,540 recombinant clones were initially generated with an average insert size of 163kb (24-fold coverage of the euchromatic portion of the genome)
    Associated Data
    Size
    Associated features
    962 Clone(s)
    Files
    Additional Information
    Synonyms and Secondary IDs (5)
    Reported As
    Symbol Synonym
    BACR
    BDGP BAC library
    RPCI-98_BAC
    Name Synonyms
    BAC genomic clone collection for D. melanogaster, iso-1 strain, adult, 163kb inserts.
    Secondary FlyBase IDs
      References (2)