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General Information
Name
DGCr2
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000780
Project
Created by
Vector
    Title
    A non-redundant collection of full length cDNA clones derived from a variety of stages and tissues.
    Accessions
    Overview
    Description

    A non-redundant collection of cDNA clones derived from a variety of developmental stages and tissues

    Available from
    [BPRC](http://bacpac.chori.org/library.php?id=239)
    [DGRC Clones](https://dgrc.bio.indiana.edu/clones/Catalog)
    Biosample Source
    Overview
    Strain
    Stage
    Sex
    Tissue isolated
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation
    Reagent Details
    Methods
    Protocol
    Mode of Assay
    Data analysis

    cDNA clones from new cDNA libraries (RH_cDNA, RE_cDNA and AT_cDNA) were analyzed together with clones from cDNA libraries analyzed in the previous DGCr1 build (LD_pBS_cDNA, LD_pOT2_cDNA, GM_pBS_cDNA, GM_pOT2_cDNA, HL_pBS_cDNA, HL_pOT2_cDNA, GH_cDNA, LP_cDNA and SD_cDNA) to generate a non-redundant set of full length cDNA clones. First, 5' ESTs from clones were sequenced and grouped by sequence to select clones that extended furthest upstream. The remaining clones were clustered on the basis of their 3' EST sequences. Duplicate clones were eliminated, as were clones lacking a poly(A) tail, and chimeric clones in which the 5' and 3' EST did not align to the genome with some proximity to each other. In this way, a total of 10,910 validated cDNA clones were selected to generate a second, more comprehensive version of the Drosophila Gene Collection.

    Comments
    Associated Data
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    Files
    Additional Information
    Synonyms and Secondary IDs (3)
    Reported As
    Symbol Synonym
    Name Synonyms
    A non-redundant collection of full length cDNA clones derived from a variety of stages and tissues.
    Secondary FlyBase IDs
      References (1)