Two independent biological replicates used.
Chromatin was immunoprecipitated using rabbit anti-Doc2 antiserum (FBrf0160877). Since Doc2 and Doc3 have almost identical expression patterns and are functionally redundant, the two proteins are expected to occupy the same sites.
Chromatin was prepared from formaldehyde-fixed embryos as previously described (FBrf0202481).
Immunoprecipitated chromatin was analyzed by Affymetrix GeneChip Drosophila Tiling 1.0R array