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General Information
Name
RNA-Seq_mE4437_AM_30d
Species
D. melanogaster
Assay type
FlyBase ID
FBlc0001346
Project
Data Provider
Title
RNA-Seq of D. melanogaster, iso-1 strain, adult male (30 days post-eclosion), modENCODE, unstranded.
Biosample Source
Overview
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation

Fly stocks were reared in bottles at 24oC. Flies were collected immediately after eclosion and aged for 29 days. Males were selected then frozen on dry ice.

Biosamples used (1)
Biosample
Type
Title
D. melanogaster, iso-1 strain, adult male (30 days post-eclosion), source for RNA.
Assay Details
Key genes
Protocol

Frozen samples were homogenized and extracted using the TRIzol reagent protocol (Invitrogen). RNA was purified on an RNeasy spin column (Qiagen), and DNase treated. Polyadenylated RNAs were purified from total RNA extracts via oligo(dT) binding, using standard Illumina protocol. The poly(A)+ RNA was fragmented using divalent cations under elevated temperature, following by first and second strand cDNA synthesis primed with random hexamers. The cDNA fragments were end-repaired using T4 DNA polymerase and Klenow DNA polymerase, and phosphorylated at their 5' ends with T4 polynucleotide kinase. After adding A bases to the 3' end of the DNA fragments, Illumina adaptor oligonucleotides were ligated to the ends and ~ 300 bp fragments were isolated from an agarose gel, enriched by PCR amplification, and gel-purified again.

Mode of Assay

Read length (bases):76

The samples were quantitated using a Nanodrop, and loaded onto a flow cell for cluster generation and sequenced on an Illumina Genome Analyzer II using either single read or paired end protocols (Illumina).

Comments
Associated Data
Size
Files
Additional Information
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
RNA-Seq_mE4437_AM_30d
Name Synonyms
RNA-Seq of D. melanogaster, iso-1 strain, adult male (30 days post-eclosion), modENCODE, unstranded.
Secondary FlyBase IDs
    References (7)