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General Information
Name
RNA-Seq_mE4289_A_4d_Zn_4.5mM
Species
D. melanogaster
Assay type
FlyBase ID
FBlc0001392
Project
Data Provider
Title
RNA-Seq of D. melanogaster, Oregon-R-modENCODE strain, adult (4 day), 4.5mM zinc treatment.
Biosample Source
Overview
Sex
Tissue isolated
Other tissues studied
Cell component
Cell line
Key genes
Sample preparation

Newly eclosed adults were aged for 2 days, then exposed to 4.5mM ZnCl[[2]] (in 10% sucrose solution) for 48 hours; 39.4% lethality was observed. Survivors were flash frozen in liquid nitrogen and stored at -80oC prior to RNA preparations.

Biosamples used (1)
Biosample
Type
Title
D. melanogaster, Oregon-R-modENCODE strain, adult (4 day), 4.5mM zinc treatment, source for RNA.
Assay Details
Key genes
Protocol

Total RNA was isolated using Trizol, then Qiagen RNeasy spin columns. mRNA was isolated using Dynal oligo(dT) beads, then fragmented using divalent cations under elevated temperature, followed by sequential ligation of RNA linkers to the 5’ and 3’ ends. Next, reverse transcription was performed using a primer complementary to the 3’ linker and PCR was performed using primers complementary to both linkers. ~ 300 bp fragments were isolated from an agarose gel and gel-purified again.

Mode of Assay

Read length (bases):76

The samples were quantitated using a Nanodrop, and loaded onto a flow cell for cluster generation and sequenced on an Illumina Genome Analyzer II using either single read or paired end protocols (Illumina).

Comments
Associated Data
Size
Files
Additional Information
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
RNA-Seq_mE4289_A_4d_Zn_4.5mM
Name Synonyms
RNA-Seq of D. melanogaster, Oregon-R-modENCODE strain, adult (4 day), 4.5mM zinc treatment.
Secondary FlyBase IDs
    References (2)