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General Information
Name
CAGE-Seq_mE5348_L3_12hr
Species
D. melanogaster
Assay type
FlyBase ID
FBlc0001442
Project
Data Provider
Title
CAGE-Seq of D. melanogaster, iso-1 strain, larva (L3, 12hr post-molt), modENCODE.
Biosample Source
Overview
Sex
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation

Adult fly population cages were maintained at 24 ̊C on a 24-hour light cycle (14 hours light/10 hours dark). After a 2hr pre-lay, embryos were collected on agar plates for a 2 hour interval during a light cycle and allowed to hatch. Larvae were allowed to feed until 83-85hr AEL, then frozen on dry ice.

Biosamples used (1)
Biosample
Type
Title
D. melanogaster, iso-1 strain, larva (L3, 12hr post-molt), source for RNA.
Assay Details
Key genes
Protocol

Total RNA was primed for first strand cDNA synthesis using a random N15 primer in the presence of trehalose and sorbitol. The RNA/cDNA hybrid was purified, then capped RNA was biotinylated and purified using streptavidin beads. The first strand cDNA was ligated to another primer at the 5' end for second strand cDNA synthesis. The resulting double-stranded cDNA was cleaved using EcoP15I and a second linker was ligated to the 3' end of the ds-cDNA products. 5'-CAGE tags were purified by streptavidin then amplified by PCR.

Mode of Assay

Library was sequenced using Illumina platform.

Read length (bases):36 (27nt after 9nt adapter trimmed)

Comments
Associated Data
Size
Files
Additional Information
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
CAGE-Seq_mE5348_L3_12hr
Name Synonyms
CAGE-Seq of D. melanogaster, iso-1 strain, larva (L3, 12hr post-molt), modENCODE.
Secondary FlyBase IDs
    References (2)