FB2021_01, released Feb 18, 2021

Assay: ChIP-chip_BDTNP_Kr_E4-5

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General Information
Name
ChIP-chip_BDTNP_Kr_E4-5
Species
D. melanogaster
Assay type
ChIP-chip
FlyBase ID
FBlc0001595
Project
Data Provider
Title
ChIP-chip of Kr in D. melanogaster, embryo (stage 4-5).
Accessions
    Biosample Source
    Overview
    Strain
    Stage
    blastoderm stage
    (Li et al., 2008)
    Sex
    Tissue isolated
    organism
    (Li et al., 2008)
    Other tissues studied
    Cell component
    nucleus
    (Li et al., 2008)
    Cell line
    Key genes
    Methods
    Sample preparation

    Embryos were collected aged appropriately, then dechorionated and formaldehyde fixed. Fixed embryos were then homogenized, nuclei were pelleted, and chromatin was sheared by sonication.

    Biosamples used (1)
    Biosample
    Type
    Title
    BS_BDTNP_E4-5_Kr_ChIP
    whole organism
    D. melanogaster, embryo (stage 4-5), source for chromatin (Kr ChIP).
    Assay Details
    Methods
    Key genes
    (bait_protein)
    Protocol

    Chromatin was immunoprecipitated using the appropriate antibody and the recovered material was amplified by PCR.

    Mode of Assay

    The recovered material was characterized by Affymetrix whole-genome tiling array.

    Comments
    Associated Data
    Size
    Files
    Additional Information
    Synonyms and Secondary IDs (2)
    Reported As
    Symbol Synonym
    ChIP-chip_BDTNP_Kr_E4-5
    Name Synonyms
    ChIP-chip of Kr in D. melanogaster, embryo (stage 4-5).
    (Li et al., 2008)
    Secondary FlyBase IDs
      References (2)