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General Information
Name
DamID-chip_GSE22069_Act5C_Kc167
Species
D. melanogaster
Assay type
FlyBase ID
FBlc0001761
Project
Data Provider
Title
DamID-chip of D. melanogaster, Kc167 cell line, Act5C-Dam.
Accessions
Biosample Source
Overview
Strain
Stage
Sex
Tissue isolated
Other tissues studied
Cell component
Key genes
(transgene_used)
Sample preparation

Cells were grown in BPYE medium with 25% bactopeptone, 20% yeast extract and 5% heat inactivated fetal calf serum. Expression plasmids for Dam-fusion and Dam-only proteins were transfected in parallel by electroporation, and cells were isolated 24 hr later. The Dam enzyme deposits stable adenine-methylation footprings at genomic sites bound by the Dam-fusion.

Biosamples used (1)
Biosample
Type
Title
D. melanogaster, Kc167 cell line, source for Act5C DamID.
Assay Details
Methods
Key genes
(bait_protein)
Protocol

Genomic DNA was isolated from Kc167 cells carrying the DamID construct and DNA regions that were methylated by the Dam-fusion were specifically amplified and labeled with a fluorescent dye.

Mode of Assay

Isolated DNA was hybridized to NimbleGen Drosophila 385k array rel4 300bp genomic tiling array.

Comments
Associated Data
Size
Files
Additional Information
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
DamID-chip_GSE22069_Act5C_Kc167
Name Synonyms
DamID-chip of D. melanogaster, Kc167 cell line, Act5C-Dam.
Secondary FlyBase IDs
    References (2)