Each purified bait clone (DPiM_cDNA_bait_cDNA) was used to transiently transfect S2R+ cells in a 54ml culture. The individual S2R+ cultures were induced to produce FLAG-HA fusion protein by treatment with media containing 0.35 mM Cu2SO4 for 20 hours. Cells were lysed in buffer containing protease inhibitor and passed through a 0.45um durapore filter.
