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General Information
Name
TI_set_Mi{Trojan-GAL4}.GDP
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0003378
Project
Created by
Vector
    Title
    A set of insertions using one of the Minos-based gene trap constructs Mi{Trojan-GAL4.0}, Mi{Trojan-GAL4.1} or Mi{Trojan-GAL4.2}.
    Accessions
      Overview
      Description

      A set of transgenic insertion stocks containing a gene trap cassette, derived from Mi{MIC} insertions in coding introns using Recombinase-Mediated Cassette Exchange (RMCE); see http://flypush.imgen.bcm.tmc.edu/pscreen/technique.html. The gene trap cassette contains a Trojan GAL4 exon composed of a splice acceptor, T2A peptide, GAL4 coding sequence and an Hsp70 transcription termination signal. When inserted in a coding intron in the correct orientation, the cassette should result in truncation of the trapped gene product and expression of GAL4 under the control of the regulatory sequences of the trapped gene. Each insertion line contains one of three versions of the cassette (Mi{Trojan-GAL4.0}, Mi{Trojan-GAL4.1} or Mi{Trojan-GAL4.2}), depending on the frame required to generate a gene trap.

      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Protocol
      Mode of Assay
      Data analysis

      A PCR assay was used to to identify RMCE events that inserted the cassette in the correct orientation. Potential lines were then crossed to UAS-2x-EGFP and their expression was evaluated with anti-GFP antibodies to identify gene trap lines.

      Comments
      Associated Data
      Size
      Associated features
      631 Insertion(s)
      Files
      Additional Information
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      TI_set_Mi{Trojan-GAL4}.GDP
      Name Synonyms
      A set of insertions using one of the Minos-based gene trap constructs Mi{Trojan-GAL4.0}, Mi{Trojan-GAL4.1} or Mi{Trojan-GAL4.2}.
      Secondary FlyBase IDs
        References (1)