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General Information
Name
Dps_BAC_Retrofit
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0003379
Project
Created by
Vector
    Title
    A set of over 60 interchromosomal duplications derived from site-specific integration of retrofitted BACs.
    Accessions
      Overview
      Description

      A set of over 60 interchromosomal duplications derived from site-specific integration of retrofitted BACs, generated for the Bloomington Stock Center collection. BAC clones were 'retrofitted' using the plasmid TKVBL-w+ so that they were competent for Pacman style transgenesis, and inserted by phiC31-mediated recombination into the VK33 docking site on the third chromosome.

      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Reagent collection used
      Insertion used
      Protocol

      Existing BAC clones were first 'retrofitted' using the plasmid TKVBL-w+ so that they were competent for Pacman style transgenesis, as per Kondo and Perrimon, 2009 (FBrf0209351). The retrofitted clones were then integrated into the VK33 docking site on the third chromosome using phiC31-mediated recombination as per Venken KJ et al., 2010 (FBrf0212670).

      Mode of Assay
      Data analysis
      Comments
      Associated Data
      Size
      Associated features
      61 Aberration(s)
      Additional Information
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Dps_BAC_Retrofit
      retrofitted duplications
      Name Synonyms
      A set of over 60 interchromosomal duplications derived from site-specific integration of retrofitted BACs.
      Secondary FlyBase IDs
        References (1)