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General Information
Name
TRiP-KO
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0003382
Project
Created by
Vector
    Title
    A set of transgenic constructs for sgRNA-targeted gene mutation.
    Accessions
      Overview
      Description
      A set of transgenic constructs that each consist of a short guide RNA (sgRNA) targeting the coding sequence of a gene. The sgRNA is under the control of a U6:3 (ubiquitous) promoter. When crossed to a stock that contains a source of Cas9, cleavage and mutagenesis of the targeted gene is induced, resulting in a small deletion or insertion mutation.
      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Protocol
      pCFD3 and pCFD4 vectors described at [CRISPR fly design](http://www.crisprflydesign.org/plasmids/)
      Mode of Assay
      Data analysis
      Comments
      Many of the TRiP RNAi and sgRNA stocks generated at Harvard Medical School (HMS lines) and Tsinghua University (HMC lines), but not stocks generated at the National Institute of Genetics in Japan (HMJ lines), contain the sev21 allele as a result of introducing a mutated X chromosome from Bloomington stock 35781 (FBst0035781) or 32261 (FBst0032261) as the transgenes were being established in stock.
      Associated Data
      Size
      Associated features
      2,115 Transgenic Construct(s)
      2,054 oligo(s)
      Files
      Additional Information
      Synonyms and Secondary IDs (2)
      Reported As
      Name Synonyms
      A set of transgenic constructs for sgRNA-targeted gene mutation.
      Secondary FlyBase IDs
        References (2)