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General Information
Name
CRIMIC-TG4
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0003479
Project
Created by
Vector
    Title
    A set of flies containing a CRIMIC Trojan-GAL4 gene trap cassette, inserted via CRISPR/Cas9-induced homology directed repair.
    Accessions
      Overview
      Description

      A set of stocks that each contain a Trojan-GAL4 gene trap sequence in a coding intron, inserted via CRISPR/Cas9-induced homology directed repair. The inserted cassette contains a 'Trojan GAL4' gene trap element composed of a splice acceptor site followed by the T2A peptide, the GAL4 coding sequence and an SV40 polyadenylation signal. When inserted in a coding intron in the correct orientation, the cassette should result in truncation of the trapped gene product and expression of GAL4 under the control of the regulatory sequences of the trapped gene. Each line contains one of three versions of the cassette (TI{CRIMIC.TG4.0}, TI{CRIMIC.TG4.1} or TI{CRIMIC.TG4.2}), depending on the frame required to generate a gene trap. In addition, the presence of inverted attP sites flanking the inserted DNA allows for the entire cassette to be replaced with DNA from a compatible donor plasmid (where the sequence to be inserted is flanked by inverted attB sites) through recombination-mediated cassette exchange (RMCE) driven by the phiC31:int integrase.

      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Protocol
      Mode of Assay
      Data analysis

      A PCR assay was used to to validate CRISPR/Cas9-induced homology directed repair events.

      Comments
      Associated Data
      Size
      Associated features
      762 Insertion(s)
      Files
      Additional Information
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CRIMIC-TG4
      Name Synonyms
      A set of flies containing a CRIMIC Trojan-GAL4 gene trap cassette, inserted via CRISPR/Cas9-induced homology directed repair.
      Secondary FlyBase IDs
        References (1)