First data version provided (2018-09-24) has been deprecated.
This data update incorporates newer FlyBase gene model anntotations, information on genomic variants overlapping sgRNA designs, and a new machine learning prediction score.
At FlyBase, these sgRNA designs are viewable in JBrowse, sorted into five different JBrowse tracks based on their specificity (predicted off-target matches): 1. no off-target sites (high stringency), 2. no off-target sites (medium stringency), 3. no off-target sites (low stringency), 4. 1-3 off-target sites (low stringency), 5. more than 3 off-target sites and/or at least 1 off-target site in a CDS (low stringency).
For additional information on overlappng genomic variants and off-target sites, use the sgRNA design's sequence to search the DRSC CRISPR tool (http://www.flyrnai.org/crispr/).