Open Close
General Information
Name
EG_pGEMT_cDNA
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0003489
Project
Created by
Shuji Shigenobu, Okazaki National Research Institutes
Vector
Title
EG cDNA library of D. melanogaster, embryonic gonadal cells purified by virture of EGFP-vas expression, pGEMT-Easy vector.
Accessions
Overview
Description
Available from
Biosample Source
Overview
Strain
Sex
Tissue isolated
Other tissues studied
Cell component
Cell line
Key genes
Sample preparation
Fly gonads were isolated from embryos carrying EGFP-vasa transgene that express GFP only in pole cells by using fluorescence-activated cell sorting (FACS).
Biosamples used (1)
Biosample
Type
Title
D. melanogaster, purified embryonic gonadal cells, source for cDNA.
Reagent Details
Methods
Molecular Construct used
Protocol
Total RNA was extracted from the isolated gonads at embryonic stages 13-16 by using RNeasy Mini kit (QIAGEN). Poly(A)+ RNA was purified Oligotex dT30 (TaKaRa) from the total RNA. cDNA was synthesized and amplified by Long-Distance PCR using SMART technology (Clontech) and cloned into pGEMT-Easy Easy vector (Promega). cDNA inserts are flanked by linker sequences 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' (5' linker: SMART IV Oligonucleotide, Clontech) and 5'-NBAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACATGTCGGCCGCCTCGGCCTCTAGAAT-3' (3' linker: CDSIII/3' PCR Primer, Clontech).
Mode of Assay
Data analysis
Comments
Associated Data
Size
Associated features
5,284 Clone(s)
Files
Additional Information
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Name Synonyms
EG cDNA library of D. melanogaster, embryonic gonadal cells purified by virture of EGFP-vas expression, pGEMT-Easy vector.
Secondary FlyBase IDs
  • FBlc0000045
References (1)