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General Information
Name
HD_CFD
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0003497
Project
    Created by
    [CRISPR fly design](http://www.crisprflydesign.org/)
    Vector
      Title
      The Heidelberg CRISPR Fly Design Library (HD_CFD) collection; a set of transgenic constructs for conditional sgRNA-targeted gene mutation.
      Accessions
        Overview
        Description

        A set of transgenic constructs that each contain UAS regulatory sequences driving expression of two different sgRNAs, both of which target common coding exons within the 5' half of a single gene of interest without overlapping the start codon; this is designed to result in an indel (small insertion or deletion mutation) of the target gene in the presence of a source of Cas9. Designed for conditional CRISPR/Cas9 mutagenesis; a GAL4 driver can be used to express a HD_CFD construct and a UAS-Cas9 transgene in a tissue-specific manner, inducing mutagenesis only in the GAL4-expressing cells.

        Available from
        Biosample Source
        Overview
        Strain
        Stage
        Sex
        Tissue isolated
        Other tissues studied
        Cell component
        Cell line
        Key genes
        Methods
        Sample preparation
        Reagent Details
        Methods
        Protocol
        Mode of Assay
        Data analysis
        Comments
        Associated Data
        Size
        Associated features
        1,634 Transgenic Construct(s)
        3,268 oligo(s)
        Files
        Additional Information
        Synonyms and Secondary IDs (3)
        Reported As
        Symbol Synonym
        HD_CFD
        Heidelberg CRISPR Fly Design Library
        Name Synonyms
        The Heidelberg CRISPR Fly Design Library (HD_CFD) collection; a set of transgenic constructs for conditional sgRNA-targeted gene mutation.
        Secondary FlyBase IDs
          References (1)