FlyBase Recombinant Construct Report: pP{CaSpeR}

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Recombinant Construct: pP{CaSpeR}

General Information

Symbol

pP{CaSpeR}

FlyBase ID

FBmc0000168

Feature type

engineered_plasmid

Component Allele(s)

Size

Expression Data

Associated insertion(s)

Molecular map

Description and Uses

Construct components

Sequence Data

Sequence (FB)

FlyBase-compiled

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

(bases 1-7775 )

Segments and Size

Total Size

Left end

HindIII (inclusive)

(Pirrotta, 1988)

P5\' (inclusive) (stated as revision)

(FlyBase, 1992-)

Right end

HindIII

(Pirrotta, 1988)

P5\' (exclusive) (stated as revision)

(FlyBase, 1992-)

Segments

Num

Orientation

Symbol

1

+

CaSpeR_P5'(P5',Sp)

1

+

2

+

3

+

Car1_P3'(H3,P3')

4

+

Reference

(FlyBase, 1992-)

Phenotypic Data

Component Allele

Phenotypic Data

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

(Pirrotta, 1988)

(Bier et al., 1989)

descendant(s)

(Pirrotta, 1994.7.13)

(Pirrotta, 1988)

(Chen et al., 1998)

(Kassis, 1994)

P{GAL4-nanos.NGT}

(Bloomington Drosophila Stock Center, 1998.9.14)

P{GAL4-slbo.2.6}

(Rorth et al., 1998)

(White et al., 1996)

P{lacZ-nanos3'UTR}

(Gavis and Lehmann, 1992)

(Kassis, 1994)

(Kassis, 1994)

(Kassis, 1994)

(Kassis, 1994)

(Kassis, 1994)

(Kassis, 1994)

(Kassis, 1994)

(Kassis, 1994)

(Kassis, 1994)

(Kassis, 1994)

(Kassis, 1994)

(Tomkiel et al., 1995)

(Reichhart and Ferrandon, 1998.6.8)

pP{Antp-lacZ.0.6d9}

(Boulet and Scott, 1988)

pP{Antp-lacZ.2d9}

(Boulet and Scott, 1988)

pP{Antp-lacZ.4d9.w}

(Boulet and Scott, 1988)

(Ito et al., 1997)

(Thummel and Pirrotta, 1991)

(Thummel and Pirrotta, 1991)

(Thummel and Pirrotta, 1991)

pP{CaSpeR(NotI)}

(Malicki et al., 1993)

pP{CaSpeR-act(B)}

(Thummel et al., 1988)

pP{CaSpeR-act(R)}

(Thummel et al., 1988)

pP{CaSpeR-AUG-βgal}

(Thummel et al., 1988)

pP{CaSpeR-Hsp70/SV40}

(Bourgouin et al., 1992)

pP{CaSpeRlacZ.M}

(Margolis et al., 1994)

pP{CaSpeR-αTub84B}

(Meng et al., 1999)

pP{CaSpeR-βgal}

(FlyBase, 1992-)

(Larochelle et al., 1998)

(Larochelle et al., 1998)

(Wharton and Crews, 1993, Wharton, 1991)

(Wharton and Crews, 1993, Wharton, 1991)

(Padgett et al., 1993)

(Padgett et al., 1993)

(Goralski et al., 1989)

(Munroe et al., 1998.5.12)

(Schejter and Shilo, 1989)

(Kassis, 1990)

pP{en.96-HZ73(w)}

(Vincent et al., 1990)

(Kassis et al., 1991)

(Kassis, 1990)

(Kassis, 1990)

(Kassis, 1990)

(Kassis et al., 1991)

(Kassis et al., 1991)

(Kassis, 1990)

(Kassis, 1990)

(Kassis, 1990)

(Kassis et al., 1991)

(Kassis et al., 1991)

(Kassis, 1990)

(Kassis, 1990)

pP{GAL4-elav.L}

(Luo et al., 1994)

(Castelli-Gair et al., 1994)

pP{GAL4-twi.BS}

(Baker and Schubiger, 1996)

pP{HS:Ubx-Antp}

(Mann and Hogness, 1990)

(Mann and Hogness, 1990)

(Mann and Hogness, 1990)

(Mann and Hogness, 1990)

pP{HS:Ubx-IVaFS}

(Mann and Hogness, 1990)

pP{HS:Ubx-ΔNN}

(Mann and Hogness, 1990)

pP{HS:Ubx-ΔSN}

(Mann and Hogness, 1990)

(Tsai and Gergen, 1994)

(Zhang and Odenwald, 1995)

(Grether et al., 1995)

pP{Hsp70-CaSpeR}

(Bell et al., 1991)

(Juni et al., 1996)

(Kassis et al., 1992)

pP{MHC.5'.3'.WT}

(Hodges et al., 1999)

(Warmke et al., 1992)

(Warmke et al., 1992)

(Whiteley and Kassis, 1997)

(Kim-Ha et al., 1991)

(Rorth, 1994)

(Rorth, 1994)

pP{snf⁺,dhd⁺}

(Larochelle et al., 1998)

(Parkhurst et al., 1988)

(Parkhurst et al., 1988)

(Krueger et al., 1996)

(Rorth, 1998)

(Wohlwill and Bonner, 1991)

(Gloor et al., 1991)

Comments

construct_comment: One of a series of constructs based upon pP{CaSpeR} and differing in cloning sites.

(Thummel and Pirrotta, 1991)

report comment: Compiled sequence is alternate view: in opposite orientation relative to sequence entry X81644 | 551447 | 29 Sep 1994; sequence discrepancies.

(FlyBase, 1992-)

report comment: Sequence discrepancies: see report for component segment w^+mC4.1(Sp).

(Pirrotta, 1988)

construct_comment: NOTE: A map of this construct can be viewed on the Web page "P-element vector maps" at http://www-hhmi.genetics.utah.edu/thummel/pelement.html.

(Thummel, 1996.8.8)

report comment: Compiled sequence is an alternate view: in opposite orientation relative to sequence entry. Included as non-attributed correction.

(Thummel and Pirrotta, 1991)

construct_comment: Good map of pP{CaSpeR} shown in Figure 5 in FBrf0068634.

(Petersen et al., 1994)

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CaSpeR_vector

(Pirrotta, 1988)

pCaSpeR

(FlyBase, 1996-)

pCaSpeR 1

(FlyBase, 1996-)

pP{CaSpeR-1}

(FlyBase, 1996-)

pP{CaSpeR}

(FlyBase, 1996-, Pirrotta, 1994.9.14)

Secondary FlyBase IDs

References (54)

Report Sections