Recombinant construct pP{CaSpeR-4}

General Information
Symbol	pP{CaSpeR-4}	FlyBase ID	FBmc0000178
Feature type	engineered_construct

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference plasmid
Uses	CV term Qualifiers & info Reference cloning vector transposon \| P-element (Thummel and Pirrotta, 1991)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent X81645 (bases 1-7862)
Segments & Size
Total Size
Left end	HindIII (inclusive) (Thummel and Pirrotta, 1991) P5\' (inclusive) (stated as revision) (FlyBase, 1992-)
Right end	HindIII (Thummel and Pirrotta, 1991) P5\' (exclusive) (stated as revision) (FlyBase, 1992-)

Segments	Number Orientation Symbol 1 + CaSpeR_P5'(P5',Sp) 1 + pCaSpeR-mcs 2 + pW8mcs+H3 3 + Car1_P3'(H3,P3') 4 + pCar1-P
Reference	(FlyBase, 1992-)
Features
	CV term Qualifiers & info Reference
Component Alleles

Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{CaSpeR} (Thummel and Pirrotta, 1991)
descendant(s)	P{3xP3-ECFPaf} (Horn and Wimmer, 2000) P{3xP3-EYFPaf} (Horn and Wimmer, 2000) P{Ace.Fb} (Incardona and Rosenberry, 1996) P{BigParent} (Lu et al., 2001) P{CaSpeR-4} (Thummel and Pirrotta, 1991) P{corto⁺9} (Kodjabachian et al., 1998) P{Crew} (Siegal and Hartl, 1996) P{GAL4-dpp.H} (Hazelett et al., 1998) P{GAL4-Eh.0.8} (McNabb et al., 1997) P{GAL4-Eh.2.4} (McNabb et al., 1997) P{hs-lacZ.-194:+84.w} (Wu et al., 2001) P{loxP(w^+mC)} (Siegal and Hartl, 1996) P{SmallParent} (Lu et al., 2001) P{thr⁺11} (D'Andrea et al., 1993) P{tubP-GAL4} (Lee and Luo, 1999) P{tubP-GAL80} (Lee and Luo, 1999) P{w⁺cos1-5} (Kerrebrock et al., 1995) P{w⁺cos1-12} (Kerrebrock et al., 1995) P{w⁺cos4-4} (Kerrebrock et al., 1995) pP{3xP3-EGFP} (Horn et al., 2000) pP{C4PLZ} (Wharton and Crews, 1993) pP{CaSpeR4-lo-} (Roman and Davis, 2002) pP{CaSpeR4-lo+} (Roman and Davis, 2002) pP{CaSpeR-5} (Le et al., 2007) pP{CaSpeR-lacZ} (Thummel and Pirrotta, 1991) pP{CXY21} (Harrison and Perrimon, 1993) pP{EP} (Rorth, 1996) pP{EPg} (Mata et al., 2000) pP{f⁺13} (Petersen et al., 1994) pP{GAL4-btl.S} (Shiga et al., 1996) pP{GAL4-nos.UTR} (Van Doren, 1997.3.14) pP{GAL4-per.BS} (Kaneko, 1997.3.14) pP{GAL4-twi.G} (Greig and Akam, 1993) pP{Germ70} (Serano et al., 1994) pP{Germ80} (Serano et al., 1994) pP{Germ90} (Serano et al., 1994) pP{Mae-UAS.E} (Rorth, 1996) pP{Mae-UAS.E^S} (Rorth, 1996) pP{Mae-UAS.EP^S} (Rorth, 1996) pP{OW10.0} (Garfinkel et al., 1992) pP{PTGAL} (Sharma et al., 2002) pP{RDC4} (Bellen et al., 1992) pP{RIC4} (Bellen et al., 1992) pP{RMC4} (Bellen et al., 1992) pP{RNC4} (Bellen et al., 1992) pP{RRC4} (Bellen et al., 1992) pP{UASp-Cer} (Lund et al., 2011) pP{UASp-Dendra2} (Lund et al., 2011) pP{UASp-GFP} (Lund et al., 2011) pP{UASp-mCh} (Lund et al., 2011) pP{UASp-paGFP} (Lund et al., 2011) pP{UASp-Ven} (Lund et al., 2011) pP{Ubi-GFP.nls} (Davis et al., 1995) pP{Ubi-GFP} (Davis et al., 1995) pP{V2-Cer} (Lund et al., 2011) pP{V2-Dendra2} (Lund et al., 2011) pP{V2-GFP} (Lund et al., 2011) pP{V2-mCh} (Lund et al., 2011) pP{V2-paGFP} (Lund et al., 2011) pP{V2-Ven} (Lund et al., 2011) pP{wFl} (Siegal and Hartl, 1996)
Comments
	construct_comment: NOTE: A map of this construct can be viewed on the Web page "P-element vector maps" at http://www-hhmi.genetics.utah.edu/thummel/pelement.html. (Thummel, 1996.8.8) construct_comment: Good map of pP{CaSpeR} shown in Figure 5 in FBrf0068634. (Petersen et al., 1994) report comment: Compiled sequence is alternate view: in opposite orientation relative to sequence entry X81645 \| 551448 \| 29 Sep 1994; sequence discrepancies. (FlyBase, 1992-) report comment: Compiled sequence is an alternate view: in opposite orientation relative to sequence entry. (Thummel and Pirrotta, 1991) construct_comment: One of a series of constructs based upon pP{CaSpeR} and differing in cloning sites. (Thummel and Pirrotta, 1991)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	pCaSpeR_4 (Thummel and Pirrotta, 1991) pP{CaSpeR-4} (Pirrotta, 1994.9.14, FlyBase, 1996-)
Secondary FlyBase IDs

References ( 7 )
Research paper	Lund et al., 2011, Fly 5(3): 255--260 A set of P-element transformation vectors permitting the simplified generation of fluorescent fusion proteins in Drosophila melanogaster. [FBrf0214747] Le et al., 2007, BioTechniques 42(2): 164, 166 CaSpeR5, a family of Drosophila transgenesis and shuttle vectors with improved multiple cloning sites. [FBrf0200750] Petersen et al., 1994, Genetics 136(1): 173--182 forked proteins are components of fiber bundles present in developing bristles of Drosophila melanogaster. [FBrf0068634] Thummel and Pirrotta, 1992, D. I. S. 71: 150 Technical notes: new pCasper P-element vectors. [FBrf0082699] Thummel and Pirrotta, 1991, D. I. N. 2: Technical Notes: New pCaSpeR P-element vectors. [FBrf0053508]
Personal communication to FlyBase	Thummel, 1996.8.8, P-element vector maps. P-element vector maps. [FBrf0087820]
DNA/RNA sequence record	Pirrotta, 1994.9.14, GenBank/EMBL/DDBJ: X81645 D.melanogaster P element CaSpeR-4 gene. [FBrf0172391]