Recombinant construct pBacΔSac

General Information
Symbol	pBacΔSac	FlyBase ID	FBmc0002758
Feature type	engineered_construct	Created / Updated	2001-04-24/2007-05-10

Size		Expression data
Location-dependent role		Associated insertions	0 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference plasmid
Uses	CV term Qualifiers & info Reference transposase source source immobile (Handler and Harrell, 1999) Tni\piggyBac\T \| active in germ-line (Horn et al., 2000) Tni\piggyBac\T \| source immobile (Horn et al., 2000)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference
Component Alleles

Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments
	construct_comment: Tested in D. melanogaster; phsp-pBac found to be more effective than pBacΔSac. (Horn et al., 2000) construct_comment: Low efficiency in D. melanogaster; phsp-pBac approx. 8-fold more efficient. (Handler and Harrell, 1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	pBacΔSac (Horn et al., 2000, Handler and Harrell, 1999, FlyBase, 1996-)
Secondary FlyBase IDs

References ( 3 )
Research paper	Horn et al., 2000, Dev. Genes Evol. 210(12): 623--629 Highly sensitive, fluorescent transformation marker for Drosophila transgenesis. [FBrf0132292] Handler and Harrell, 1999, Insect Molec. Biol. 8(4): 449--457 Germline transformation of Drosophila melanogaster with the piggyBac transposon vector. [FBrf0123037]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]