Recombinant construct pSacBII

General Information
Symbol	pSacBII	FlyBase ID	FBmc0002975
Feature type	engineered_construct

Size	16.011	Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference P1 vector
Uses	CV term Qualifiers & info Reference cloning vector phage P1 \| component (Hartl et al., 1994)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent U09128 (bases 1-16011)
Segments & Size
Total Size	16.011 (Hartl et al., 1994)
Left end	BamHI (inclusive) (Hartl et al., 1994)
Right end	BamHI (Hartl et al., 1994)

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference
Component Alleles

Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)	pBACe3.6 (FlyBase, 1992-)
Comments
	construct_comment: Equivalent to P1(pAd10-sacBII) without the Ad10 stuffer fragment (after loxP recombination). (Hartl et al., 1994) construct_comment: Portion of P1 cloning vector P1(pAd10-sacBII) packaged after insertion of cloned DNA. Equivalent to P1(pAd10-sacBII) without the Ad10 stuffer fragment (after loxP recombination).Insertion in BamHI cloning site prevents expression of sacBII, allowing selection in high concentrations of sucrose.Reference: Pierce et al., 1992, Proc. Natl. Acad. Sci. USA 89:2056-2060. (Hartl et al., 1994)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	pAd (BDGP Project Members, 1994-1999) pSacBII (Roe, 1994.4.20, FlyBase, 1996-)
Secondary FlyBase IDs
	FBtp0001097
References ( 3 )
Research paper	Hartl et al., 1994, Proc. Natl. Acad. Sci. U.S.A. 91(15): 6824--6829 Genome structure and evolution in Drosophila: applications of the framework P1 map. [FBrf0073296]
Personal communication to FlyBase	BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
DNA/RNA sequence record	Roe, 1994.4.20, GenBank/EMBL/DDBJ: U09128 pSacBII P1 cloning vector with sacB, kilA, repL and kanamycin resistance genes, complete sequence. [FBrf0172533]