Recombinant construct pKC26

General Information
Symbol	pKC26	FlyBase ID	FBmc0003025
Feature type	engineered_construct

Size	4.805	Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference plasmid
Uses	CV term Qualifiers & info Reference cloning vector recombination-mediated genomic insertion \| phiC31\attP (Keleman et al., 2009.8.5)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	4.805 (Keleman et al., 2009.8.5)
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference
Component Alleles

Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments
	For production of dsRNA, contains 10 Scer\GAL4-responsive Scer\UAS elements, the basal Hsp70Bb promoter, and the SV40 polyadenylation signal. For genomic recombination, contains an phiC31\attB sequence to allow for phiC31-targeted integration at genomic phiC31\attP landing sites, and the 5' portion of a mini-white gene; regeneration of the complete mini-white occurs upon integration into appropriate target element. (Keleman et al., 2009.8.5) Map and sequence may be found at http://www.vdrc.at/fileadmin/conferences/VDRC/KK_protocols. (Keleman et al., 2009.8.5) Cloning vector used for the VDRC-2 phiC31 RNAi library collection. (Keleman et al., 2009.8.5)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	pKC26 (FlyBase, 1996-, Keleman et al., 2009.8.5)
Secondary FlyBase IDs

References ( 1 )
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]