Recombinant construct pVALIUM20

General Information
Symbol	pVALIUM20	FlyBase ID	FBmc0003034
Feature type	engineered_construct

Size	7.710	Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference plasmid
Uses	CV term Qualifiers & info Reference cloning vector binary system (Ni et al., 2010.12.1) recombination-mediated genomic insertion \| phiC31\attP (Ni et al., 2010.12.1) RNAi (Ni et al., 2010.12.1)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	7.710 (Ni et al., 2010.12.1)
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference
Component Alleles

Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)	P{VALIUM20-EGFP} (Transgenic RNAi Project, 2011.6.14) P{VALIUM20-GAL4.1} (Transgenic RNAi Project, 2011.6.14) P{VALIUM20-GAL4.2} (Transgenic RNAi Project, 2011.6.14) P{VALIUM20-mCherry} (Transgenic RNAi Project, 2011.6.14)
Comments
	Efficacy of pVALIUM20-based vs pVALIUM22-based RNAi constructs in germline and soma compared. pVALIUM20 generates effective knockdown phenotypes in both germline and soma; pVALIUM22 is more effective for knockdowns in the germline and effects appear to be restricted to the female germline (excluding somatic ovarian cells), but is significantly less effective in the soma. (Ni et al., 2011) "VALIUM" stands for Vermilion-AttB-Loxp-Intron-UAS-MCS. (Ni et al., 2010.12.1) pVALIUM20 allows cloning of short hairpins as annealed synthetic oligos; these are targeted against specific genes via the production of short hairpin microRNAs. pVALIUM20 contains vermillion as a selectable marker; an attB sequence to allow phiC31-targeted integration at genomic attP landing sites; two gypsy sequences to enhance hairpin DNA transcription; two pentamers of UAS, one of which can be excised using the Cre/loxP system to generate a 5XUAS derivative; the hsp70 basal promoter; a multiple cloning site (MCS) for cloning shmiRNAs in a miR1 scaffold, and a ftz 3'UTR intron followed by a SV40 3'UTR as a source for a polyA signal sequence. (Ni et al., 2010.12.1) pVALIUM20 gives a stronger knockdown than pVALIUM10 in the soma, and works well in the germline. (Ni et al., 2010.12.1) Map and additional information at http://www.flyrnai.org/TRiP-TTR-GEN2.html. (Ni et al., 2010.12.1)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	pVALIUM20 (FlyBase, 1996-) Valium20 (Ni et al., 2011)
Secondary FlyBase IDs

References ( 3 )
Research paper	Ni et al., 2011, Nat. Methods 8(5): 405--407 A genome-scale shRNA resource for transgenic RNAi in Drosophila. [FBrf0213581]
Personal communication to FlyBase	Transgenic RNAi Project, 2011.6.14, Control RNAi constructs and insertions from the Transgenic RNAi Project. Control RNAi constructs and insertions from the Transgenic RNAi Project. [FBrf0213920] Ni et al., 2010.12.1, A genome-scale shRNA resource for transgenic RNAi in Drosophila. A genome-scale shRNA resource for transgenic RNAi in Drosophila. [FBrf0212437]