Recombinant construct pVALIUM22

General Information
Symbol	pVALIUM22	FlyBase ID	FBmc0003035
Feature type	engineered_construct

Size	8.433	Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference plasmid
Uses	CV term Qualifiers & info Reference cloning vector binary system (Ni et al., 2010.12.1) recombination-mediated genomic insertion \| phiC31\attP (Ni et al., 2010.12.1) RNAi (Ni et al., 2010.12.1)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	8.433 (Ni et al., 2010.12.1)
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference
Component Alleles

Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments
	Efficacy of pVALIUM20-based vs pVALIUM22-based RNAi constructs in germline and soma compared. pVALIUM20 generates effective knockdown phenotypes in both germline and soma; pVALIUM22 is more effective for knockdowns in the germline and effects appear to be restricted to the female germline (excluding somatic ovarian cells), but is significantly less effective in the soma. (Ni et al., 2011) "VALIUM" stands for Vermilion-AttB-Loxp-Intron-UAS-MCS. (Ni et al., 2010.12.1) pVALIUM22 allows cloning of short hairpins as annealed synthetic oligos; these are targeted against specific genes via the production of short hairpin microRNAs. pVALIUM22 contains vermillion as a selectable marker; an attB sequence to allow phiC31-targeted integration at genomic attP landing sites; two gypsy sequences to enhance hairpin DNA transcription; two pentamers of UAS, one of which can be excised using the Cre/loxP system to generate a 5XUAS derivative; the P-transposase core promoter; a multiple cloning site (MCS) for cloning shmiRNAs in a miR1 scaffold, and a ftz 3'UTR intron followed by a K10 polyA. (Ni et al., 2010.12.1) pVALIUM22 triggers effective RNAi knock-down and corresponding phenotypes in the female germline; it is less effective in somatic cells. (Ni et al., 2010.12.1) Map and additional information at http://www.flyrnai.org/TRiP-TTR-GEN2.html. (Ni et al., 2010.12.1)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	pVALIUM22 (FlyBase, 1996-) Valium22 (Ni et al., 2011)
Secondary FlyBase IDs

References ( 2 )
Research paper	Ni et al., 2011, Nat. Methods 8(5): 405--407 A genome-scale shRNA resource for transgenic RNAi in Drosophila. [FBrf0213581]
Personal communication to FlyBase	Ni et al., 2010.12.1, A genome-scale shRNA resource for transgenic RNAi in Drosophila. A genome-scale shRNA resource for transgenic RNAi in Drosophila. [FBrf0212437]