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General Information
Symbol
pVALIUM22
FlyBase ID
FBmc0003035
Feature type
engineered_plasmid
Component Allele(s)
Size
8.973
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Description

pVALIUM22 allows cloning of short hairpins as annealed synthetic oligos, targeted against specific genes via the production of short hairpin microRNAs; contains v+t1.8 as a selectable marker; the P-transposase core promoter; a multiple cloning site (MCS) for cloning shRNAs in a mir-1 scaffold; a ftz 3'UTR intron followed by a fs(1)K10 3'UTR to facilitate shRNA processing and export from the nucleus; two pentamers of UAS, one of which is flanked by loxP sites and can thus be excised using the P1cre recombinase to generate a 5xUAS derivative; two gypsy insulator sequences flanking the transcription unit to enhance hairpin DNA transcription; an attB sequence to allow phiC31:int-targeted integration at genomic attP landing sites.

Construct components
Product class / Tool use(s)
Marker(s)
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
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Right end
 
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Symbol
Reference
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

Efficacy of pVALIUM20-based vs pVALIUM22-based RNAi constructs in germline and soma compared. pVALIUM20 generates effective knockdown phenotypes in both germline and soma; pVALIUM22 is more effective for knockdowns in the germline and effects appear to be restricted to the female germline (excluding somatic ovarian cells), but is significantly less effective in the soma.

See TRiP(http://fgr.hms.harvard.edu/trip-plasmid-vector-sets) for downloadable maps, sequences and cloning protocols.

'VALIUM' stands for Vermilion-AttB-Loxp-Intron-UAS-MCS. pVALIUM22 triggers effective RNAi knockdown and corresponding phenotypes in the female germline; it is less effective in somatic cells.

Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Valium22
pVALIUM22
Secondary FlyBase IDs
    References (4)