vector is designed for expression of two guide RNA (gRNA) sequences, with each sgRNA being expressed individually under the control of a ubiquitous promoter; the two promoters are U6:1 (snRNA:U6:96Aa
) and U6:3 (snRNA:U6:96Ac
). The vector contains an attB
site, allowing integration into an attP
-containing docking site in the genome to produce a transgenic construct.