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General Information
Symbol
pDEST-APIC-Cas9
FlyBase ID
FBmc0003180
Feature type
engineered_construct
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
plasmid
Construct components
Product class / Tool use(s)
Encoded product / tool
Description

Gateway destination vector. Contains a synthetic core promoter followed by sequence encoding the Cas9 RNA-guided nuclease tagged with nuclear localization signals at both the N-terminal and C-terminal ends of the open reading frame. A tissue-specific enhancer can be swapped in to this vector to generate a tissue-specific Cas9 expression construct which can then be inserted into the genome using phiC31:int integrase.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
pDEST-APIC-Cas9
Secondary FlyBase IDs
    References (2)