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Citation	O'Donnell, J., Gerace, L., Leister, F., Sofer, W. (1975). Chemical selection of mutants that affect alcohol dehydrogenase in Drosophila. II. Use of 1-pentyne-3-ol. Genetics 79(): 73--83.
FlyBase ID	FBrf0027526
Type of publication	Research paper
Offprint Available	Yes
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PubMed ID	1126622
PubMed Abstract	We describe a procedure for the selection of alcohol dehyrogenase negative mutants in Drosophila. The method consists of exposing eggs and larvae to low concentrations of 1-pentyne-3-ol dissolved in the culture medium. Only those flies with greatly reduced levels of alcohol dehydrogenase activity survive. In addition, genotypically negative flies die if their mothers are alcohol dehydrogenase positive. Using this procedure and formaldehyde to generate mutants, we were able to detect seven alcohol dehydrogenase negative mutants out of 350,000 individuals subjected to selection. At least five of the mutants contain small deletions that include the alcohol dehydrogenase locus.
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Language of publication	English
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Abbreviation	Genetics
Title	Genetics
Authors
Volume range	1-
Year range	1916-
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Place of publication	Austin, TX, etc
Language of publication	English
ISBN/ISSN	0016-6731
CODEN	GENTAE
Data from Reference
Aberrations (1)
	In(2LR)O
Alleles (15)
	Adh^fn23 Adh^fn24 Adh^fn4 Adh^fn6 Adhⁿ¹⁰ Adhⁿ¹¹ Adhⁿ¹² Adhⁿ¹³ Adhⁿ¹⁴ Adhⁿ⁶ Adhⁿ⁷ Adhⁿ⁸ Adhⁿ⁹ Adh^nA Adh^nB
Balancers (3)
	CyO CyO-vOa CyO-vOb
Genes (1)
	Adh