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Reference
Citation
Gehring, W.J., Klemenz, R., Weber, U., Kloter, U. (1984). Functional analysis of the white+ gene of Drosophila by P-factor-mediated transformation.  EMBO J. 3: 2077--2085.
FlyBase ID
FBrf0040771
Publication Type
Research paper
Abstract

A 12-kb DNA segment spanning the white (w) locus of Drosophila has been inserted into a P-transposon vector and used for P-factor-mediated germ-line transformation. Several red-eyed transformants were recovered which complement the white mutant phenotype. Analysis of the eye pigments and the interaction with the zeste mutation indicates that the w gene inserted at several new chromosomal sites is expressed normally. The tissue-specific accumulation of w transcripts, as studied by in situ hybridization to tissue sections, is the same in transformant and wild-type larvae. This indicates that all the genetic information specified by the w locus is contained within this 12-kb segment of DNA. By secondary mobilization it was shown that the w sequences have been inserted as a functional P(w) transposon which is capable of further transposition.

PubMed ID
PubMed Central ID
PMC557646 (PMC) (EuropePMC)
DOI
Associated Information
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    EMBO J.
    Title
    The EMBO Journal
    Publication Year
    1982-
    ISBN/ISSN
    0261-4189
    Data From Reference
    Aberrations (1)
    Alleles (10)
    Genes (3)
    Molecular Constructs (2)
    Insertions (1)
    Transgenic Constructs (1)