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Reference
Citation
Frei, E., Baumgartner, S., Edstrom, J.E., Noll, M. (1985). Cloning of the extra sex combs gene of Drosophila and its identification by P-element mediated gene transfer.  EMBO J. 4(4): 979--987.
FlyBase ID
FBrf0042446
Publication Type
Research paper
Abstract

A large region containing the extra sex combs (esc) gene of Drosophila has been cloned by microdissection from polytene chromosomes and chromosomal walking. Within this DNA, the segment comprising the esc gene has been narrowed down in several steps. First, a deletion of the esc gene, Df(2L)esc, defined a region of 380 kb. Then, a duplication carrying the esc gene, Dp(2;2)GYL, and a deletion not eliminating esc, Df(2L)prd, further reduced this region to 160 kb. Finally, because esc transcripts are expected to be present in follicles but absent in adult males, mapping of follicle and adult male transcripts limited the esc region to 10 kb. Rigorous proof that this DNA contained the esc gene was obtained by P-element-mediated transformation. We conclude that the 12 kb of DNA used for transformation include the esc gene as well as all flanking sequences required for its proper regulation in the female germ line.

PubMed ID
PubMed Central ID
PMC554288 (PMC) (EuropePMC)
DOI
Associated Information
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    EMBO J.
    Title
    The EMBO Journal
    Publication Year
    1982-
    ISBN/ISSN
    0261-4189
    Data From Reference
    Aberrations (4)
    Alleles (6)
    Genes (2)
    Molecular Constructs (1)
    Insertions (1)
    Transgenic Constructs (1)