Reference Report

Reference
Citation	Smith, D.A., Baker, B.S., Gatti, M. (1985). Mutations in genes encoding essential mitotic functions in Drosophila melanogaster. Genetics 110(): 647--670. (Export to RIS)
FlyBase ID	FBrf0042650
Publication Type	Research paper
PubMed ID	3928429
PubMed Abstract	Temperature-sensitive mutations at 15 loci that affect the fidelity of mitotic chromosome behavior have been isolated in Drosophila melanogaster. These mitotic mutants were detected in a collection of 168 EMS-induced X-linked temperature-sensitive (ts) lethal and semilethal mutants. Our screen for mutations with mitotic effects was based upon the reasoning that under semirestrictive conditions such mutations could cause an elevated frequency of mitotic chromosome misbehavior and that such events would be detectable with somatic cell genetic techniques. Males hemizygous for each ts lethal and heterozygous for the recessive autosomal cell marker mwh were reared under semirestrictive conditions, and the wings of those individuals surviving to adulthood were examined for an increased frequency of mwh clones. Those mutations producing elevated levels of chromosome instability during growth of the wing imaginal disc were also examined for their effects on chromosome behavior in the cell lineages producing the abdominal cuticle. Fifteen mutations affect chromosome behavior in both wing and abdominal cells and thus identify loci generally required for the fidelity of mitotic chromosome transmission. Mapping and complementation tests show that these mutations represent 15 loci. One mutant is an allele of a locus (mus-101) previously identified by mutagen-sensitive mutants and a second mutant is an allele of the lethal locus zw 10.--The 15 mutants were also examined cytologically for their effects on chromosomes in larval neuroblasts. Taken together, the results of our cytological and genetical studies show that these mutants identify loci with wild-type functions necessary for either maintenance of chromosome integrity or regular disjunction of chromosomes or chromosome condensation. Thus, these mutations define a broad spectrum of genes required for the normal execution of the mitotic chromosome cycle.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (6)
	Df(1)HA92 Df(1)w-rJ1 Dp(1;3)sc^J4 T(1;3)sc^J4 T(Y;3)D8 Ts(YSt;3Lt)D8
Alleles (18)
	mit(1)2¹ mit(1)3¹ mit(1)4¹ mit(1)5¹ mit(1)6¹ mit(1)7¹ mit(1)8¹ mit(1)9¹ mit(1)10¹ mit(1)11¹ mit(1)12¹ mit(1)13¹ mit(1)14¹ mit(1)15³ mit(1)15⁵ mit(1)15¹⁵ mus101^D1 mus101^ts1
Genes (16)
	mit(1)2 mit(1)3 mit(1)4 mit(1)5 mit(1)6 mit(1)7 mit(1)8 mit(1)9 mit(1)10 mit(1)11 mit(1)12 mit(1)13 mit(1)14 mit(1)15 mus101 mwh