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Reference
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| Citation |
Martin, P.F., Place, A.R., Pentz, E., Sofer, W. (1985). UGA nonsense mutation in the alcohol dehydrogenase gene of Drosophila melanogaster. J. molec. Biol. 184(): 221--229. |
| FlyBase ID |
FBrf0043104 |
| Type of publication |
Research paper |
| Offprint Available |
Yes |
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External Crossreferences
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| PubMed ID |
3928896 |
| PubMed Abstract |
A mutant gene, which we have designated AdhnB, codes for a defective form of the enzyme alcohol dehydrogenase in Drosophila
melanogaster. We show that the polypeptide encoded by AdhnB is approximately 2000 Mr smaller than the protein synthesized
under the direction of the wild-type alcohol dehydrogenase gene. In contrast, the alcohol dehydrogenase mRNA produced by both
genes is the same size. We cloned and sequenced a portion of the protein-coding region of AdhnB and compared it to the same
region in the wild-type gene. We found a single base substitution: a change of the TGG tryptophan codon at amino acid 235
to a TGA termination codon. This nonsense mutation accounts for the observed reduction in size of the alcohol dehydrogenase
polypeptide. In further studies, we found that the steady-state levels of alcohol dehydrogenase mRNA in flies carrying the
AdhnB gene and the wild-type alcohol dehydrogenase gene were indistinguishable. However, the steady-state level of alcohol
dehydrogenase polypeptide was reduced to 1% of wild-type levels in flies with the AdhnB gene. Moreover, the rate of alcohol
dehydrogenase synthesis in mutant flies was reduced to 50% of that found in wild type. The aberration in AdhnB thus affects
both the rate of synthesis and the rate of degradation of the alcohol dehydrogenase peptide. AdhnB is the first reported nonsense
mutant in Drosophila.
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| Biosis |
80095030 |
| Zoological record |
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Associated Information
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| Comments |
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| Text of personal communication |
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Related Publications
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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| Place of publication |
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Published In
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| Abbreviation |
J. molec. Biol. |
| Title |
Journal of Molecular Biology |
| Authors |
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| Volume range |
1- |
| Year range |
1959- |
| Page range |
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| Publisher |
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| Place of publication |
London |
| Language of publication |
English |
| ISBN/ISSN |
0022-2836 |
| CODEN |
JMOBAK |
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Data from Reference
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| Alleles (1)
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| Genes (2)
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