Reference Report

Reference
Citation	Roiha, H., Rubin, G.M., O'Hare, K. (1988). P element insertions and rearrangements at the singed locus of Drosophila melanogaster. Genetics 119(): 75--83.
FlyBase ID	FBrf0048245
Type of publication	Research paper
Offprint Available	Yes
External Crossreferences
PubMed ID	2840331
PubMed Abstract	DNA from the singed gene of Drosophila melanogaster was isolated using an inversion between a previously cloned P element at cytological location 17C and the hypermutable allele singed-weak. Five out of nine singed mutants examined have alterations in their DNA maps in this region. The singed locus is a hotspot for mutation during P-M hybrid dysgenesis, and we have analyzed 22 mutations induced by P-M hybrid dysgenesis. All 22 have a P element inserted within a 700-bp region. The precise positions of 10 P element insertions were determined and they define 4 sites within a 100-bp interval. During P-M hybrid dysgenesis, the singed-weak allele is destabilized, producing two classes of phenotypically altered derivatives at high frequency. In singed-weak, two defective P elements are present in a "head-to-head" or inverse tandem arrangement. Excision of one element results in a more extreme singed bristle phenotype while excision of the other leads to a wild-type bristle phenotype.
Biosis	86048315
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Language of publication	English
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Abbreviation	Genetics
Title	Genetics
Authors
Volume range	1-
Year range	1916-
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Publisher
Place of publication	Austin, TX, etc
Language of publication	English
ISBN/ISSN	0016-6731
CODEN	GENTAE
Data from Reference
Aberrations (3)
	In(1)B332.1 In(1)B337.2 Tp(1;3)sn^13a1
Alleles (36)
	sn⁽⁺⁾ sn¹ sn² sn^34e sn^36a sn³ sn⁴ sn⁵ sn^5S sn^79b10 sn^79f22 sn^79h2 sn^83i7(1) sn^83i7(15) sn^83i7(17) sn^83i7(2) sn^83i7(3) sn^A2.4 sn^B332.1 sn^B337.2 sn^CFL3 sn^CFL5 sn^KH36 sn^KH40 sn^KHL1 sn^KHL3 sn^KHL4 sn^KHL5 sn^MH18.2 sn^MH26.5 sn^MI17 sn^MJ19.3 sn^MK7 sn^X2 sn^e sn^w
Genes (2)
	hdp-b sn
Insertions (26)
	P{}sn⁽⁺⁾ P{}sn^79b10 P{}sn^79f22 P{}sn^79h2 P{}sn^83i7(1) P{}sn^83i7(15) P{}sn^83i7(17) P{}sn^83i7(2) P{}sn^83i7(3) P{}sn^A2.4 P{}sn^CFL3 P{}sn^CFL5 P{}sn^KH36 P{}sn^KH40 P{}sn^KHL1 P{}sn^KHL3 P{}sn^KHL4 P{}sn^KHL5 P{}sn^MH18.2 P{}sn^MH26.5 P{}sn^MI17 P{}sn^MJ19.3 P{}sn^MK7 P{}sn^e P{}sn^wa P{}sn^wb
Natural transposons (1)
	P-element