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Reference
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| Citation |
Wilson, C., Pearson, R.K., Bellen, H.J., O'Kane, C.J., Grossniklaus, U., Gehring, W.J. (1989). P-element-mediated enhancer detection: an efficient method for isolating and characterizing developmentally regulated genes
in Drosophila. Genes Dev. 3(9): 1301--1313. |
| FlyBase ID |
FBrf0049802 |
| Type of publication |
Research paper |
| Offprint Available |
Yes |
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External Crossreferences
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| PubMed ID |
2558051 |
| PubMed Abstract |
We describe a new approach for identifying and studying genes involved in Drosophila development. Single copies of an enhancer
detector transposon, P[1ArB], have been introduced into flies at many different genomic locations. The beta-galactosidase
reporter gene in this construct is influenced by a wide range of genomic transcriptional regulatory elements in its vicinity.
Our results suggest that a significant proportion of these regulatory sequences are control elements of nearby Drosophila
genes. These genes need not be disrupted for their regulatory elements to be identified by P[1ArB]. The P[1ArB] transposon
has been designed to facilitate both rapid cloning and deletion analysis of genomic sequences into which it inserts. Therefore,
the enhancer detection system is an efficient method of screening for genes primarily on the basis of their expression pattern
and then rapidly analyzing those of particular interest at the molecular and genetic levels.
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| Biosis |
88119197 |
| Zoological record |
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Associated Information
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| Comments |
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| Text of personal communication |
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| Associated files |
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Related Publications
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Also Published As
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Other Reference Information
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| Secondary IDs |
FBrf0049392 |
| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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| Place of publication |
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Published In
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| Abbreviation |
Genes Dev. |
| Title |
Genes & Development |
| Authors |
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| Volume range |
1- |
| Year range |
1987- |
| Page range |
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| Publisher |
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| Place of publication |
Cold Spring Harbor, NY |
| Language of publication |
English |
| ISBN/ISSN |
0890-9369 |
| CODEN |
GEDEEP |
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Data from Reference
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| Aberrations (1)
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| Alleles (18)
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| Balancers (3)
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| Constructs (42)
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- 001005
- 001006
- 001007
- 001008
- 001008-Xb
- 001009
- 001010
- 001011
- Adh+3.2(Xb)
- BL2_mcs(Sc1,K)
- P\T::lacZ(P5',R1)b
- P{lArB}
- P{lAwB}
- P{lacZ-un5}
- P{lrB}
- P{lwB}
- pBL-S
- pBL2-S
- pBL2M-N
- pBL2M-mcs(K,Sc1)
- pBL2M-mcs(Sc1,K)
- pBL2P-N
- pBL2P-mcs(K,Sc1)
- pBL2P-mcs(Sc1,K)
- pBLM-N
- pBLM-mcs(K,Sc1)
- pBLM-mcs(Sc1,K)
- pBLP-N
- pBLP-mcs(K,Sc1)
- pBLP-mcs(Sc1,K)
- pBluescript_II_KS(+)
- pBluescript_II_KS(-)
- pBluescript_II_SK(+)
- pBluescript_II_SK(-)
- pBluescript_KS(+)
- pBluescript_KS(-)
- pBluescript_SK(+)
- pBluescript_SK(-)
- pP{lArB}
- pP{lAwB}
- pP{lrB}
- pP{lwB}
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| Genes (11)
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| Insertions (7)
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