Reference Report

Reference
Citation	Wilson, C., Pearson, R.K., Bellen, H.J., O'Kane, C.J., Grossniklaus, U., Gehring, W.J. (1989). P-element-mediated enhancer detection: an efficient method for isolating and characterizing developmentally regulated genes in Drosophila. Genes Dev. 3(9): 1301--1313. (Export to RIS)
FlyBase ID	FBrf0049802
Publication Type	Research paper
PubMed ID	2558051
PubMed Abstract	We describe a new approach for identifying and studying genes involved in Drosophila development. Single copies of an enhancer detector transposon, P[1ArB], have been introduced into flies at many different genomic locations. The beta-galactosidase reporter gene in this construct is influenced by a wide range of genomic transcriptional regulatory elements in its vicinity. Our results suggest that a significant proportion of these regulatory sequences are control elements of nearby Drosophila genes. These genes need not be disrupted for their regulatory elements to be identified by P[1ArB]. The P[1ArB] transposon has been designed to facilitate both rapid cloning and deletion analysis of genomic sequences into which it inserts. Therefore, the enhancer detection system is an efficient method of screening for genes primarily on the basis of their expression pattern and then rapidly analyzing those of particular interest at the molecular and genetic levels.
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Secondary IDs	FBrf0049392
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Aberrations (1)
	In(2LR)O
Alleles (18)
	Adh^+t3.2 Cg25C^A109.1F2 Dl^A326.2F3 Ecol\lacZ^A107.1F3 Ecol\lacZ^A107.1M3 Ecol\lacZ^A401.1M3 Ecol\lacZ^{Cg25C-A109.1F2} Ecol\lacZ^cpo-1 Ecol\lacZ^{Fas3-A183.1F2} Ecol\lacZ^{fs(3)83Ba-A33.1M3} Ecol\lacZ^P\T.A92a Fas3^A183.1F2 l(3)A164.1M3¹ l(3)A519.1F3¹ l(3)B14.1M3¹ Mi-2¹ ry^+t7.2 w^+mW.hs
Balancers (3)
	CyO CyO-CZ CyO-vWa
Constructs (42)
	001005 001006 001007 001008 001008-Xb 001009 001010 001011 Adh⁺3.2(Xb) BL2_mcs(Sc1,K) P{lacZ-un5} P{lArB} P{lAwB} P{lrB} P{lwB} P\T::lacZ(P5',R1)b pBL2M-mcs(K,Sc1) pBL2M-mcs(Sc1,K) pBL2M-N pBL2P-mcs(K,Sc1) pBL2P-mcs(Sc1,K) pBL2P-N pBL2-S pBLM-mcs(K,Sc1) pBLM-mcs(Sc1,K) pBLM-N pBLP-mcs(K,Sc1) pBLP-mcs(Sc1,K) pBLP-N pBL-S pBluescript_II_KS(-) pBluescript_II_KS(+) pBluescript_II_SK(-) pBluescript_II_SK(+) pBluescript_KS(-) pBluescript_KS(+) pBluescript_SK(-) pBluescript_SK(+) pP{lArB} pP{lAwB} pP{lrB} pP{lwB}
Genes (11)
	Adh Cg25C Dl Ecol\lacZ Fas3 l(3)A164.1M3 l(3)A519.1F3 l(3)B14.1M3 Mi-2 ry w
Insertions (7)
	P{lArB}A107.1F3 P{lArB}A107.1M3 P{lArB}A401.1M3 P{lArB}Cg25C^A109.1F2 P{lArB}cpo¹ P{lArB}Fas3^A183.1F2 P{lArB}fs(3)83Ba^A33.1M3
Transcripts (1)
	Ecol\lacZ^{Fas3-A183.1F2}RA