A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Reference Report

Reference
Citation Wilson, C., Pearson, R.K., Bellen, H.J., O'Kane, C.J., Grossniklaus, U., Gehring, W.J. (1989). P-element-mediated enhancer detection: an efficient method for isolating and characterizing developmentally regulated genes in Drosophila.  Genes Dev. 3(9): 1301--1313.
FlyBase ID FBrf0049802
Type of publication Research paper
Offprint Available Yes
External Crossreferences
PubMed ID 2558051
PubMed Abstract We describe a new approach for identifying and studying genes involved in Drosophila development. Single copies of an enhancer detector transposon, P[1ArB], have been introduced into flies at many different genomic locations. The beta-galactosidase reporter gene in this construct is influenced by a wide range of genomic transcriptional regulatory elements in its vicinity. Our results suggest that a significant proportion of these regulatory sequences are control elements of nearby Drosophila genes. These genes need not be disrupted for their regulatory elements to be identified by P[1ArB]. The P[1ArB] transposon has been designed to facilitate both rapid cloning and deletion analysis of genomic sequences into which it inserts. Therefore, the enhancer detection system is an efficient method of screening for genes primarily on the basis of their expression pattern and then rapidly analyzing those of particular interest at the molecular and genetic levels.
Biosis 88119197
Zoological record
hide Associated Information
Comments
Text of personal communication
Associated files
hide Related Publications
hide Also Published As
hide Other Reference Information
Secondary IDs FBrf0049392
Language of publication English
Additional language(s) of abstract
ISBN
Place of publication
hide Published In
Abbreviation Genes Dev.
Title Genes & Development
Authors
Volume range 1-
Year range 1987-
Page range
Publisher
Place of publication Cold Spring Harbor, NY
Language of publication English
ISBN/ISSN 0890-9369
CODEN GEDEEP
hide Data from Reference
hideAberrations (1)
hideAlleles (18)
hideBalancers (3)
hideConstructs (42)
hideGenes (11)
hideInsertions (7)