Reference Report

Reference
Citation	Hays, T.S., Deuring, R., Robertson, B., Prout, M., Fuller, M.T. (1989). Interacting proteins identified by genetic interactions: a missense mutation in -tubulin fails to complement alleles of the testis-specific -tubulin gene of Drosophila melanogaster. Mol. Cell. Biol. 9(3): 875--884. (Export to RIS)
FlyBase ID	FBrf0050548
Publication Type	Research paper
PubMed ID	2498648
PubMed Abstract	In this paper we demonstrate that failure to complement between mutations at separate loci can be used to identify genes that encode interacting structural proteins. A mutation (nc33) identified because it failed to complement mutant alleles of the gene encoding the testis-specific beta 2-tubulin of Drosophila melanogaster (B2t) did not map to the B2t locus. We show that this second-site noncomplementing mutation is a missense mutation in alpha-tubulin that results in substitution of methionine in place of valine at amino acid 177. Because alpha- and beta-tubulin form a heterodimer, our results suggest that the genetic interaction, failure to complement, is based on the structural interaction between the protein products of the two genes. Although the nc33 mutation failed to complement a null allele of B2t (B2tn), a deletion of the alpha-tubulin gene to which nc33 mapped complemented B2tn. Thus, the failure to complement appears to require the presence of the altered alpha-tubulin encoded by the nc33 allele, which may act as a structural poison when incorporated into either the tubulin heterodimer or microtubules.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Aberrations (12)
	Df(3R)Antp2 Df(3R)Antp17 Df(3R)Antp-A41 Df(3R)dsx2M Df(3R)dsx29 Df(3R)p13 Df(3R)p30 Df(3R)Scr Df(3R)Scx2 Df(3R)Scx4 Df(3R)Tpl10 Df(3R)WIN3
Alleles (9)
	αTub84B^7.tHa αTub84B⁷ αTub84B^7XH αTub84B^+t4.8 αTub84B^unspecified αTub84B^XH βTub85D³ βTub85D⁸ βTub85Dⁿ
Constructs (4)
	P{NC33} P{NC33-XH} P{WT-1} P{WT-XH}
Genes (4)
	Ki p αTub84B βTub85D