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Citation
Axton, J.M., Dombradi, V., Cohen, P.T.W., Glover, D.M. (1990). One of the protein phosphatase 1 isoenzymes in Drosophila is essential for mitosis.  Cell 63(): 33--46.
FlyBase ID
FBrf0051367
Publication Type
Research paper
Abstract

Drosophila has four loci encoding type 1 protein serine/threonine phosphatases (PP1s). Here we describe mutations in one of these genes, at 87B on chromosome 3. Mutants die at the larval-pupal boundary with little or no imaginal cell proliferation. Neuroblasts are delayed in progress through mitosis and show defective spindle organization, abnormal sister chromatid segregation, hyperploidy, and excessive chromosome condensation. Germline transformation of mutant flies with the wild-type PP1 87B gene restores normal mitosis, viability, and fertility. These results show that PP1 activity is required for mitotic progression and that the other loci cannot supply sufficient activity to complement loss of expression of the PP1 87B gene. Alternatively, the PP1 87B product may have a distinct specialized function in mitosis.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
    Data From Reference
    Aberrations (5)
    Alleles (9)
    Genes (3)
    Transgenic Constructs (2)